acrylamide ready gel zymogram gels (Bio-Rad)
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Acrylamide Ready Gel Zymogram Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 10005 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Expressing:Article Title: G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist Article Snippet: Equal amounts of protein (30 μg) were applied on a mouse VEGF-A ELISA (R&D Systems) following the manufacturer's instructions. .. Evaluation of MMP2 expression using gelatin zymography Tumor lysates (30 μg) were loaded on 10% Zymography:Article Title: G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist Article Snippet: Equal amounts of protein (30 μg) were applied on a mouse VEGF-A ELISA (R&D Systems) following the manufacturer's instructions. .. Evaluation of MMP2 expression using gelatin zymography Tumor lysates (30 μg) were loaded on 10% Incubation:Article Title: G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist Article Snippet: Equal amounts of protein (30 μg) were applied on a mouse VEGF-A ELISA (R&D Systems) following the manufacturer's instructions. .. Evaluation of MMP2 expression using gelatin zymography Tumor lysates (30 μg) were loaded on 10% Article Title: G-CSF supplementation with chemotherapy can promote revascularization and subsequent tumor regrowth: prevention by a CXCR4 antagonist Article Snippet: Equal amounts of protein (30 μg) were applied on a mouse VEGF-A ELISA (R&D Systems) following the manufacturer's instructions. .. Tumor lysates (30 μg) were loaded on 10% |
![FIG. 3. SDS-PAGE (A) and Western immunoblot (B) of secreted proteins from S. aureus RN6390 and isogenic derivatives. The molec- ular masses of protein standards are indicated to the left of each gel. (A) Lane 1, S. aureus RN6390; lane 2, SP6391; lane 3, SP6912; lane 4, RN6911. The amount of protein loaded represents the equivalent of 2.3 OD600 units (lanes 1 and 2) or 6.8 OD600 units (lanes 3 and 4) of original culture. Secreted proteins are visualized by staining with Coo- massie blue dye. (B) Purified V8 protease (60 ng) from S. aureus L530 (lane 1) or supernatant corresponding to 0.03 OD600 units of stationary phase culture from S. aureus RN6390 (lane 2), SP6391 (lane 3), SP6912 (lane 4), or RN6911 (lane 5) was subjected to SDS-PAGE (10% <t>[wtzvol21]</t> polyacrylamide), transferred to Immobilon-P membrane, and probed with blot-purified polyclonal anti-V8 protease antibody.](https://doi-unpaywalled-images-cdn.bioz.com/1522/10__1128_slash_iai__69__1__159___169__2001/10__1128_slash_iai__69__1__159___169__2001____page6_image1.jpg)
![SDS-PAGE (A) and Western immunoblot (B) of secreted proteins from S. aureus RN6390 and isogenic derivatives. The molecular masses of protein standards are indicated to the left of each gel. (A) Lane 1, S. aureus RN6390; lane 2, SP6391; lane 3, SP6912; lane 4, RN6911. The amount of protein loaded represents the equivalent of 2.3 OD600 units (lanes 1 and 2) or 6.8 OD600 units (lanes 3 and 4) of original culture. Secreted proteins are visualized by staining with Coomassie blue dye. (B) Purified V8 protease (60 ng) from S. aureus L530 (lane 1) or supernatant corresponding to 0.03 OD600 units of stationary phase culture from S. aureus RN6390 (lane 2), SP6391 (lane 3), SP6912 (lane 4), or RN6911 (lane 5) was subjected to SDS-PAGE (10% <t>[wt·vol−1]</t> polyacrylamide), transferred to Immobilon-P membrane, and probed with blot-purified polyclonal anti-V8 protease antibody.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_7868/pmc00097868/pmc00097868__ii0110915003.jpg)